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Risk, cancer and manmade chemicals

Blaming synthetic chemicals for a 'cancer epidemic' is flawed science that makes for dubious policy.

Various Authors

Topics Politics

Entering a new millennium seems a good time to challenge some old ideas about cancer cause and prevention, which in our view are implausible, have little supportive evidence, and might best be left behind. In this essay, we summarise data and conclusions from 15 years of work, raising five issues that involve toxicology, nutrition, public health, and US government regulatory policy:

1. There is no cancer epidemic other than that due to smoking.

2. The dose makes the poison. Half of all chemicals tested, whether natural or synthetic, cause cancer in high-dose rodent cancer tests. Evidence suggests that this high rate is due primarily to effects that are unique to high doses. The results of these high-dose tests have been used to regulate low-dose human exposures, but are not likely to be relevant.

3. Even Rachel Carson was made of chemicals: natural v synthetic chemicals. Human exposure to naturally occurring rodent carcinogens is ubiquitous and dwarfs the exposure of the general public to synthetic rodent carcinogens.

4. Errors of omission. The major causes of cancer (other than smoking) do not involve exposures to exogenous chemicals that cause cancer in high-dose tests; rather, the major causes are dietary imbalances, hormonal factors, infection and inflammation, and genetic factors. Insufficiency of many vitamins and minerals, which is preventable by supplementation, causes DNA damage by a mechanism similar to radiation.

5. Damage by distraction: regulating low hypothetical risks. Regulatory policy places unwarranted emphasis on reducing low-level exposures to synthetic chemicals. Putting large amounts of money into small hypothetical risks can damage public health by diverting resources and distracting the public from major risks.

The dose makes the poison

The main rule in toxicology is that ‘the dose makes the poison’. At some level, every chemical becomes toxic, but there are safe levels below that.

In contrast to that rule, a scientific consensus evolved in the 1970s that we should treat carcinogens differently, that we should assume that even low doses might cause cancer, even though we lacked the methods for measuring carcinogenic effects at low levels. In large part, this assumption was based on the idea that mutagens – chemicals that cause changes in DNA – are carcinogens and that the risk of mutations was directly related to the number of mutagens introduced into a cell. It was also assumed that: 1. only a small proportion of chemicals would have carcinogenic potential; 2. testing at a high dose would not produce a carcinogenic effect unique to the high dose; and 3. carcinogens were likely to be synthetic industrial chemicals. It is time to take account of information indicating that all three assumptions are wrong.

Laws and regulations directed at synthetic chemicals got a big push from the widely publicised ‘cancer epidemic’, which supposedly stemmed from exposures to those chemicals. In fact, there is not now and there never was a cancer epidemic, and cancer mortality, excluding lung cancer mortality, has declined by 19 percent since 1950 (1). Lung cancer mortality began dropping around 1990 as a result of reduced smoking rates, and that trend is likely to continue. Regardless of the absence of evidence for a cancer epidemic, the ‘epidemic’ has left a long-lasting legacy – a regulatory focus on synthetic chemicals.

About 50 percent of chemicals, both natural and synthetic, that have been tested in standard, high-dose, animal cancer tests are rodent carcinogens (Table 1) (2). What explains the high percentage? In standard cancer tests, rodents are given a near-toxic dose of the test substance over their lifetime, the maximum tolerated dose (MTD), to maximise the chance of detecting any carcinogenicity. Evidence is accumulating that cell division caused by the high dose itself, rather than the chemical per se, contributes to cancer in these tests (3).

High doses can cause chronic wounding of tissues, cell death, and consequent chronic cell division of neighbouring cells, which would otherwise not divide. Cell division is a risk factor for cancer because there is some probability that a mutation will occur each time DNA is replicated, and some of those mutations can lead to cancer. A high proportion (41 percent) of chemicals that are carcinogens in rodent tests are not mutagenic, and their carcinogenicity may result from cell killing and consequent division at the high doses tested. Such increased cell division does not occur at the low levels of synthetic chemicals to which humans are usually exposed.

Defenders of rodent tests argue that the high rate of positive tests results from selecting more suspicious chemicals to test, and this seems a likely bias because cancer testing is both expensive and time-consuming, making it prudent to test suspicious compounds. One argument against such a selection bias is the high rate of positive tests for drugs (Table 1) because drug development favours chemicals that are not mutagens or expected carcinogens (4).

A second argument against selection bias is that the knowledge needed to predict carcinogenicity in rodent tests is highly imperfect, even now, after decades of test results have become available on which to base predictions. For example, in 1990 there was wide disagreement among experts about which chemicals would be carcinogenic when subsequently tested by the US National Toxicology Program (5). Moreover, if the primary basis for selection of chemicals to test were suspicion of carcinogenicity, selection would focus on mutagens (80 percent are carcinogenic compared to 50 percent of nonmutagens). In fact, a majority of tested chemicals, 55 percent, are nonmutagens.

It seems likely that a high proportion of all chemicals, whether synthetic or natural, would be ‘carcinogens’ if administered in the standard rodent bioassay at the MTD, primarily because of the effects of high doses on cell death and division and DNA damage and repair (6). Without additional data about how a chemical causes cancer, the interpretation of a positive result in a rodent bioassay is highly uncertain. The induction of cancer could be the result of the high doses tested and have no predictive value about what might occur at lower doses.

The processes of mutagenesis and carcinogenesis are complicated because of many factors, which are dose-dependent (7). For instance, normal cells contain an appreciable level of DNA lesions, and they contain enzymes that repair the lesions with high efficiency (8). The number of those lesions increases in tissues injured by high doses of chemicals (9) and may overwhelm the capacity of the repair enzymes. The far lower levels of chemicals to which humans are exposed through water pollution or synthetic pesticide residues on food are not sufficient to increase the number of DNA lesions in any appreciable way and may pose no or minimal cancer risks.

Regulatory agencies do not consider the great uncertainties in extrapolating from the effects observed in high-dose rodent tests to predictions of possible effects in humans at far lower doses. Instead, they assume that the effects are directly proportional to dose – that there is a linear relationship between dose and cancer – and they calculate the ‘virtually safe dose’ (VSD), which corresponds to a maximum, hypothetical risk of one additional cancer in a million exposed people, and set the VSD as the acceptable exposure level. To the extent that high doses of nonmutagens are the cause of carcinogenicity in rodent bioassays, the linear model is inappropriate (10).

Linearity of dose response seems unlikely in any case even for chemicals that are mutagens because of the inducibility of the numerous defence enzymes that deal with the thousands of exogenous chemicals that we encounter in our diets, and protect us against the natural world of mutagens as well as the small amounts of synthetic chemicals (11).

Regulatory agencies are moving to take nonlinearity and questions about mechanisms of carcinogenicity into account; for example, the US Environmental Protection Agency (EPA) recently concluded that chloroform (a by-product of disinfecting water with chlorine) was not likely to be carcinogenic to humans unless the exposures were high enough to cause cell toxicity and increased cell division. The chloroform levels in drinking water are low and do not produce such effects (12).

Even Rachel Carson was made of chemicals: Natural v synthetic chemicals

About 99.9 percent of the chemicals humans ingest are natural, and the amounts of synthetic pesticide residues in foods are insignificant compared to the amount of natural pesticides that are always in our diet because of the plants we eat (13). Of all dietary pesticides that humans eat, 99.99 percent are natural chemicals produced by plants to defend themselves against fungi, insects, and other animal predators. The natural pesticides come in great variety because each plant produces a different array of such chemicals.

We have estimated that on average Americans ingest roughly 5,000 to 10,000 different natural pesticides and their breakdown products. Each day, the average American eats about 1,500 milligrams (mg = 1/1000th of a gram) of natural pesticides, which is about 10,000 times more than the 0.09 mg they consume of synthetic pesticide residues (14).

Only a small proportion of natural pesticides have been tested for carcinogenicity, but 38 of the 72 tested are rodent carcinogens. As shown in Table 2, naturally occurring pesticides that are rodent carcinogens are ubiquitous in common fruits, vegetables, herbs, and spices. The widespread distribution of such chemicals means that no diet can be free of natural chemicals that are rodent carcinogens.

The average American eats about 2,000 mg of burnt material, which is produced in usual cooking practices, each day. That burnt material contains many rodent carcinogens and mutagens, swamping, again, the 0.09 mg of 200 synthetic chemicals, primarily synthetic pesticides, that are ingested each day and that are classified as rodent carcinogens.

The natural chemicals that are known rodent carcinogens in a single cup of coffee are about equal in weight to a year’s worth of ingested synthetic pesticide residues that are rodent carcinogens. This is so, even though only three percent of the natural chemicals in roasted coffee have been adequately tested for carcinogenicity (Table 3). This does not mean that coffee or natural pesticides are dangerous; rather, assumptions about high-dose animal cancer tests for assessing human risk at low doses need re-examination.

Ranking risks

Gaining a broad perspective about the vast number of chemicals to which humans are exposed can be helpful when setting research and regulatory priorities. Rodent cancer tests by themselves provide little information about how a chemical causes cancer or about low-dose risk. The assumption that synthetic chemicals are hazardous has led to a bias in testing, and such chemicals account for 76 percent (451 of 590) of the chemicals tested chronically in both rats and mice (Table 1). The world of natural chemicals has never been tested systematically.

One reasonable strategy to use the available information about cancer risk is to construct an index to compare and rank possible carcinogenic hazards from a wide variety of chemical exposures at levels typically experienced by humans, and then to focus research and regulatory efforts on those that rank highest (15).

Although one cannot say whether the ranked chemical exposures are likely to be of major or minor importance in human cancer, it is not prudent to focus attention on risks at the bottom of a ranking if the same methodology identifies numerous, common human exposures that pose much greater possible risks. Our rankings are based on the human exposure/rodent potency (HERP) index, which is the ratio between the average human exposure to a chemical and the dose that caused cancer in 50 percent of exposed rodents.

Overall, our analyses have shown that HERP values for some historically high exposures in the workplace – to butadiene and tetrachloroethylene – and to some pharmaceuticals -clofibrate – rank high, and that there is an enormous background of naturally occurring rodent carcinogens in typical portions of common foods. The background of natural exposures casts doubt on the relative importance of low-dose exposures to residues of synthetic chemicals such as pesticides. (A committee of the National Research Council of the National Academy of Sciences reached similar conclusions about natural v synthetic chemicals in the diet, and called for further research on natural chemicals.) (16)

The possible carcinogenic hazards from synthetic pesticides are minimal compared to the background of nature’s pesticides, though neither may be a hazard at the low doses consumed. Analysis also indicates that many ordinary foods would not pass the regulatory criteria used for synthetic chemicals. Caution is necessary in drawing conclusions about the occurrence in the diet of natural chemicals that are rodent carcinogens. These dietary exposures are not necessarily of much relevance to human cancer. The data call for a reevaluation of the utility of animal cancer tests in protecting the public against minor hypothetical risks without understanding how the chemical causes tumours.

Cellular defences against chemical carcinogens work against natural and synthetic chemicals

It is often assumed that because natural chemicals are part of human evolutionary history, whereas synthetic chemicals are recent, the mechanisms evolved in animals to cope with the toxicity of natural chemicals will fail to protect against synthetic chemicals. This assumption is flawed for several reasons.

1. Human defences to ward off effects of exposures to toxins are usually general, directed at classes of similar chemicals, rather than tailored for specific chemicals, and they work against both natural and synthetic chemicals (17). Examples of general defences include the continuous shedding of cells exposed to toxins. The surface layers of the mouth, esophagus, stomach, intestine, colon, skin, and lungs are discarded every few days; DNA repair enzymes repair DNA damage regardless of the source of the damage. Detoxification enzymes of the liver and other organs generally react with classes of chemicals rather than individual chemicals.

General defence mechanisms make good evolutionary sense for animals, such as humans, which eat plants and encounter a diverse and ever-changing array of plant toxins in an evolving world. A herbivore that had defences against only a specific set of toxins would be at great disadvantage in obtaining new food when favoured foods became scarce or evolved new chemical defences.

2. Various natural toxins, which have been present throughout vertebrate evolutionary history, nevertheless cause cancer in vertebrates. Mould toxins, such as aflatoxin, have been shown to cause cancer in rodents (Table 1) and other species including humans. Many common elements are carcinogenic to humans at high doses – for example, salts of cadmium, beryllium, nickel, chromium, and arsenic, despite their presence throughout evolution. Furthermore, epidemiological studies from various parts of the world show that certain ingested natural substances may be carcinogenic risks to humans. Naturally occurring arsenic in drinking water causes cancer of the lung, bladder, and skin (18), and the chewing of betel nut with tobacco causes oral cancer.

3. Humans have not had time to evolve a ‘toxic harmony’ with all of their dietary plants.The human diet has changed markedly in the past few thousand years. Indeed, very few of the plants that humans eat today, such as coffee, cocoa, tea, potatoes, tomatoes, corn, avocados, mangoes, olives, and kiwi fruit, would have been present in a hunter-gatherer’s diet. Natural selection works far too slowly for humans to have evolved specific resistance to the food toxins in these newly introduced plants.

4. DDT is often viewed as the typically dangerous synthetic pesticide because it concentrates in adipose tissues and persists for years. DDT, the first synthetic pesticide, eradicated malaria from many parts of the world, including the USA. It was effective against many vectors of disease such as mosquitoes, tsetse flies, lice, ticks, and fleas and against many crop pests, significantly increasing the supply and lowering the cost of food, making fresh, nutritious foods more accessible to poor people. DDT was also of low toxicity to humans. DDT prevented many millions of deaths due to malaria (19).

There is no convincing epidemiological evidence (20), nor is there much toxicological plausibility, that the levels of DDT normally found in the environment or in human tissues are likely to be a significant contributor to cancer. Two chemical properties of DDT were important in focusing attention on it. DDT, once ingested, is stored in fatty tissues, and the DDT in an insect, when eaten by a small bird, will be concentrated and stored in the bird’s fat. If a larger bird, such as an eagle, eats the small bird, it will ingest the concentrated DDT and each additional meal of DDT-containing prey will increase the concentration. The chlorine components (substituents) of DDT cause it to be resistant to degradation in nature, and, as a result, it persists longer than most chemicals. Few synthetic chemicals share these properties.

These properties are not unique to synthetic chemicals. Many thousands of chlorinated chemicals are produced in nature (21), and natural pesticides can bioconcentrate if they are fat-soluble. Potatoes, for example, contain solanine and chaconine, which are fat-soluble, neurotoxic, natural pesticides that can be detected in the blood of all potato eaters. High levels of these potato neurotoxins have been shown to cause birth defects in rodents (22), though they have not been tested for carcinogenicity.

5. Because no plot of land is immune to attack by insects, plants need chemical defences – either natural or synthetic – to survive, and trade-offs between naturally occurring and synthetic pesticides are possible. One consequence of disproportionate concern about synthetic pesticide residues is that some plant breeders develop plants to be more insect-resistant, which sometimes increases their levels of natural pesticides, which can bring its own hazards.When a major grower introduced a new variety of highly insect-resistant celery into commerce, people who handled the celery developed rashes when they went out into the sunlight. Some detective work found that the pest-resistant celery contained 6,200 parts per billion (ppb) of carcinogenic (and mutagenic) psoralens instead of the 800 ppb present in common celery (23).

Errors of omission

High consumption of fruits and vegetables is associated with a lowered risk of degenerative diseases including cancer, cardiovascular disease, cataracts and brain dysfunction (24). More than 200 studies in the epidemiological literature show, with consistency, an association between low consumption of fruits and vegetables and high cancer incidence (Table 4). The evidence of a protective effect of fruits and vegetables is most convincing for cancers of the oral cavity, esophagus, stomach, and lung. The median relative risk of cancer of the lung, larynx, oral cavity, esophagus, stomach, bladder, pancreas, and cervix was about double for the quarter of the population with the lowest dietary intake of fruits and vegetables when compared to the quarter with the highest intake (25). The median relative risk, although elevated, was not as high for the hormonally related cancers of breast, prostate, and ovary, or for the colon.

Inadequate diets, with too few fruits and vegetables, are common. Fully 80 percent of children and adolescents (26) and 68 percent of adults (27) do not eat the five servings of fruits and vegetables per day recommended by the US National Cancer Institute and the National Research Council. Publicity about hundreds of minor hypothetical risks, such as pesticide residues, can cause a loss of perspective about what is important. In a survey, half the US public did not name fruit and vegetable consumption as protective against cancer (28).

Laboratory studies of vitamin and mineral inadequacy associate such deficiencies with DNA damage, which indicates that the vitamin and mineral content of fruits and vegetables may explain the observed association between fruit and vegetable intake and cancer risk. Antioxidants such as vitamin C (whose dietary source is fruits and vegetables), vitamin E, and selenium protect against oxidative damage caused by normal metabolism (29), smoking (30), and inflammation (31).

Laboratory evidence ranging from likely to compelling indicates that deficiency of some vitamins and minerals – folic acid, vitamins B12, B6, C, and E, niacin, iron, and zinc – causes damage to DNA that mimics the damage caused by radiation (32). In the USA, the percentage of the population that consumes less than half the Recommended Daily Allowance (RDA) in the diet (that is, ignoring supplement use) for five of these eight vitamins or minerals is estimated to be: zinc (10 percent of women/men older than 50), iron (25 percent of menstruating women, and 5 percent of women over 50), vitamin C (25 percent of women/men), folate (50 percent of women; 25 percent of men), vitamin B6 (10 percent of women/men), vitamin B12 (10 percent of women; 5 percent of men) (33).

Folic acid (or folate) deficiency, one of the most common vitamin deficiencies in the population consuming few dietary fruits and vegetables, causes chromosome breaks in humans (34), analogous to those caused by radiation. Folate supplementation above the RDA has been shown to minimise chromosome breakage (35). Researchers conducting a long-term study of women’s health, the Nurses’ Health Study, associated folate deficiency with increased risk of colon cancer (36). They also reported that women who took a multivitamin supplement containing folate for 15 years had a 75 percent lower risk of colon cancer (37). Folate deficiency also damages human sperm (38), causes neural tube defects in the fetus, and an estimated 10 percent of USA heart disease (39).

Approximately 10 percent of the US population (40) had a lower folate level than that at which chromosome breaks occur (41). The recent decision in the United States to supplement flour, rice, pasta, and cornmeal with folate (42) may reduce the percentage of the population with the deficiency.

Other vitamins – vitamin B6 and niacin – complement folic acid. Vitamin B6 deficiency apparently causes chromosome breaks by the same mechanism as folate deficiency (43). Niacin is important to the repair of DNA strand-breaks (44). As a result, dietary insufficiencies of niacin (15 percent of some populations are deficient) (45), folate, vitamin B6, and antioxidants, such as vitamin C, may interact synergistically to adversely affect DNA synthesis and repair.

People with diets deficient in fruits and vegetables generally have vitamin and mineral deficiencies. The findings summarised in Table 4, which associate higher cancer rates with such diets, underline the importance of fruits and vegetables and the vitamins and minerals they contain in cancer prevention.

Vitamins and minerals, whose main dietary sources are other than fruits and vegetables, are also likely to play a significant role in the prevention and repair of DNA damage, and thus are important to the maintenance of long-term health. Vitamin B12 is found in animal products, and deficiencies of B12 cause a functional folate deficiency, accumulation of the amino acid homocysteine (a risk factor for heart disease) (46), and chromosome breaks. B12 supplementation above the RDA was necessary to minimise chromosome breakage (47). Strict vegetarians are at increased risk for developing vitamin B12 deficiency.

Epidemiological studies of supplement usage (vitamin and mineral intake by pill) have shown at most only modest support for an association between intake of these substances and lower cancer rates. Many problems complicate those studies, including the difficulty in measuring supplement use over a long period of time, and potential confounding of supplement usage with many other aspects of a healthy lifestyle that are related to it, such as more exercise, better diet, and not smoking. Clinical trials of supplements are generally too short to measure cancer risk, since cancers usually develop slowly and the risk increases with age; moreover, such trials cannot measure the potential reduction in risk if supplements are taken throughout a lifetime.

Additionally, cancer risks of supplement users may be overestimated because they are more likely to undergo early screening like mammograms or tests for prostate cancer, which are associated with increased diagnosis rates, and can artificially increase the apparent incidence rate. Such confounding factors are not measured in many epidemiological studies.

The strongest effect in clinical trials was for a protective effect of vitamin E against cancers of the prostate and colon (48). More well-done trials will increase the information about the usefulness of supplements in cancer prevention. In the meantime, it is clear that intake of adequate amounts of vitamins and minerals may have a major effect on health, and the costs and risks of a daily multivitamin/mineral pill are low (49). More research in this area, as well as efforts to improve diets, should be high priorities for public policy.

Damage by distraction: Regulating low hypothetical risks

Synthetic chemicals that mimic hormones – ‘environmental estrogens’ or ‘endocrine disruptors’ – arose as a major environmental issue in the 1990s. Environmental concerns have focused on exposures to estrogenic organochlorine residues (largely plastics and pesticides) that are tiny compared to the normal dietary intake of naturally occurring endocrine-active chemicals in fruits and vegetables (50). These low levels of human exposure to the synthetic chemicals seem toxicologically implausible as a significant cause of cancer or of reproductive abnormalities.

Recent epidemiological studies have found no association between organochlorine pesticides and breast cancer, including one in which DDT, DDE, dieldrin, and chlordane were measured in blood of women on Long Island (51). Synthetic hormone mimics have been proposed as a cause of declining sperm counts, even though it has not been shown that sperm counts are declining (52). An analysis of US data about sperm counts found distinct geographical differences, with the highest concentrations in New York City (53). When geographic differences were taken into account, there was no significant change in sperm counts for the past 50 years.

Some recent studies have compared estrogenic equivalents (EQ) of dietary intake of synthetic chemicals v phytoestrogens (estrogens of plant origin) in the normal diet, by considering both the amounts consumed by humans and estrogenic potency. Results support the idea that synthetic residues are orders of magnitude lower in EQ and are generally weaker in potency. Scientists using a series of in vitro assays calculated the EQs in 200 ml of Cabernet Sauvignon wine and the EQs from average daily intake of organochlorine pesticides (54). EQs in a single glass of wine were about 1,000 times higher (55).

Conclusions

Because there is no risk-free world and resources are limited, society must set priorities based on cost-effectiveness in order to save the most lives (56). The EPA projected in 1991 that the cost to society of US environmental regulations in 1997 would be about US$140 billion per year (about 2.6 percent of gross national product) (57). Most of this cost is borne by the private sector, which passes much of it along to consumers in higher prices.

Several economic analyses have concluded that current expenditures are not cost-effective; that is, resources are not used so as to save the most lives per dollar. One estimate is that the USA could prevent 60,000 deaths per year by redirecting the same dollar resources to more cost-effective programmes (58). For example, the median toxin control programme, such as those administered by EPA, costs 146 times more per year of life saved than the median medical intervention programme. The true difference is likely to be greater, because cancer risk estimates for toxin control programmes are worst-case, hypothetical estimates, and there may be no risk at low dose. Rules on air and water pollution are necessary (for example, it was a public health advance to phase lead out of gasoline), and clearly, cancer prevention is not the only reason for regulations.

The many worst-case assumptions built into cancer risk assessments are there because of policy decisions, not because of scientific ones, and they confuse attempts to allocate money effectively for public health. For example, EPA estimates of synthetic pesticide residues in the diet have used the theoretical maximum human residue that is anticipated under the most severe field application conditions, which is often a large overestimate compared to the measured residues in food. Despite the EPA’s estimated high risks from exposures to several pesticides, the US Food and Drug Administration detected no residues of those pesticides in the food samples in its Total Diet Study (59).

Regulatory efforts to reduce low-level human exposures to synthetic chemicals because they are rodent carcinogens are expensive, can do nothing but reduce already minuscule chemical concentrations, and are unlikely to have any effect on cancer rates. Moreover, they distract from the major task of improving public health through increasing scientific understanding about how to prevent cancer, and increasing public understanding of how lifestyle influences health.

This is an edited version of a chapter titled ‘Cancer Prevention and the Environmental
Chemical Distraction’, by Bruce Ames and Lois Swirsky Gold, in Politicizing Science: the Alchemy of Policymaking, Michael Gough ed., Hoover Institute Press, Stanford, California (2003).

(1) L. A. G. Ries et al., SEER Cancer Statistics Review, 1973-1997 (Bethesda, Md.: National Cancer Institute, 2000).

(2) L. S. Gold et al., Misconceptions About the Causes of Cancer, L. S. Gold and E. Zeiger, eds., Handbook of Carcinogenic Potency and Genotoxicity Databases (Boca Raton, Fla.: CRC Press, 1997); L. S. Gold et al., ‘Supplement to the Carcinogemic Potency Database (CPDB): Results of Animal Bioassays Published in the General Literature in 1993-1994 and by the National Toxi- cology Programin 1995-1996,’ Environ. Health Perspect. 107 (Suppl. 4, 1999): 527-600.

(3) B. N. Ames and L. S. Gold, ‘Chemical Carcinogenesis: Too Many Rodent Carcinogens,’ Proc. Natl. Acad. Sci. U.S.A. 87 (1990): 7772-76; S. M. Cohen, ‘Cell Proliferation and Carcinogenesis,’ Drug Metab. Rev. 30 (1998): 339-57.

(4) See L. S. Gold, T. H. Slone, and B. N. Ames, ‘What Do Animal Cancer Tests Tell Us About Human Cancer Risk?: Overview of Analyses of the Carcinogenic Potency Database,’ Drug Metab. Rev. 30 (1998): 359-404.

(5) G. S. Omenn, S. Stuebbe, and L. B. Lave, ‘Predictions of Rodent Carcinogenicity Testing Results: Interpretation in Light of the Lave-Omenn Value-of-Information Model,’ Mol. Carcinog. 14 (1995): 37-45.

(6) B. Butterworth, R. Conolly, and K. Morgan, ‘A Strategy for Establishing Mode of Action of Chemical Carcinogens as a Guide for Approaches to Risk Assessment,’ Cancer Lett. 93 (1995): 129-46.

(7) J. G. Christensen, T. L. Goldsworthy,and R. C. Cattley, ‘Dysregulation of Apoptosis by C-myc in Transgenic Hepatocytes and Effects of Growth Factors and Nongenotoxic Carcinogens,’ Mol. Carcinog. 25 (1999): 273-84.

(8) H. J. Helbock et al., ‘DNA Oxidation Matters: The HPLC-EC Assay of 8-oxo-deoxyguanosine and 8-oxo-guanine,’ Proc. Natl. Acad. Sci. U.S.A. 95 (1998): 288-93.

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(10) D. W. Gaylor and L. S. Gold, ‘Regulatory Cancer Risk Assessment Based on a Quick Estimate of a BenchmarkDose Derivedfrom theMaximum Tolerated Dose,’ Regul. Toxicol. Pharmacol. 28 (1998): 222-25.

(11) T. D. Luckey, ‘Nurture with Ionizing Radiation: A Provocative Hypothesis,’ Nutr. Cancer 34 (1999): 1-11; Ames and Gold, ‘Paracelsus to Parascience.’

(12) U.S. Environmental Protection Agency, ‘Integrated Risk Information System (IRIS)’ (Cincinnati: Office of Health and Environmental Assessment, Environmental Criteria and Assessment Office, 2002).

(13) B. N. Ames, M. Profet, and L. S. Gold, ‘Nature’s Chemicals and Synthetic Chemicals: Comparative Toxicology,’ Proc. Natl. Acad. Sci. U.S.A. 87 (1990): 7782-86; B. N. Ames, M. Profet, and L. S. Gold, ‘Dietary Pesticides 99.99 Percent All Natural,’ ibid., 7777-81; L. S. Gold, T. H. Slone, and B. N. Ames, ‘Prioritization of Possible Carcinogenic Hazards in Food,’ in D. Tennant, ed., Food Chemical Risk Analysis (London: Chapman & Hall, 1997), pp. 267-95.

(14) E. L. Gunderson, ‘Chemical Contaminants Monitoring: FDA Total Diet Study, April 1982-April 1984, Dietary Intakes of Pesticides, Selected Elements, and Other Chemicals,’ J. Assoc. Off. Anal. Chem. 71 (1988):1200- 9.

(15) L. S. Gold et al., MisconceptionsAbout the Causes of Cancer; B. N. Ames, R. Magaw, and L. S. Gold, ‘Ranking Possible Carcinogenic Hazards,’ Science 236 (1987): 271-80.

(16) National Research Council, Carcinogens and Anticarcinogens in the Human Diet: A Comparison of Naturally Occurring and Synthetic Substances (Washington, D.C.: National Academy Press, 1996).

(17) Ames, Profet, and Gold, ‘Nature’s Chemicals and Synthetic Chemicals.’
18. National Research Council, Arsenic in Drinking Water: 2001 Update (Washington, D.C.: National Academy Press, 2001).

(19) National Academy of Sciences, U.S.A., The Life Sciences: Recent Progress and Application to Human Affairs, the World of Biological Research, Requirement for the Future (Washington, D.C.: Committee on Research in the Life Sciences, 1970).

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(22) Ames, Profet, and Gold, ‘Nature’s Chemicals and Synthetic Chemicals.’

(23) S. F. Berkley et al., ‘Dermatitis in Grocery Workers Associated with High Natural Concentrations of Furanocoumarins in Celery,’ Ann. Intern. Med. 105 (1986): 351-55.
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(26) S. M. Krebs-Smith et al., ‘Fruit and Vegetable Intakes of Children and Adolescents in the United States,’ Arch. Pediatr. 150 (1996): 81-86.

(27) S. M. Krebs-Smith et al., ‘U.S. Adults’ Fruit and Vegetable Intakes, 1989 to 1991: A Revised Baseline for the Healthy People 2000 Objective,’ Am. J. Public Health 85 (1995): 1623-29.

(28) National Cancer Institute Graphic, ‘Why Eat Five?’ J. Natl. Cancer Inst. 88 (1996): 1314.

(29) H. J. Helbock et al.,’DNAOxidation Matters:TheHPLC-Electrochemical Detection Assay of 8-oxo-deoxyguanosine and 8-oxo-guanine,’ Proc. Natl. Acad. Sci. USA 95 (1998): 288-93.

(30) B. N. Ames, ‘Micronutrients Prevent Cancer and Delay Aging,’ Toxicol. Lett. 103 (1998): 5-18.

(31) Ames, Shigenaga, and Hagen, ‘Oxidants, Antioxidants, and the Degenerative Diseases of Aging.’

(32) Ames, ‘Micronutrients Prevent Cancer and Delay Aging.’

(33) B. N. Ames and P. Wakimoto, ‘Are Vitamin and Mineral Deficiencies a Major Cancer Risk?’ Nature Rev. Cancer 2 (2002): 694-704.

(34) B. C. Blount et al., ‘Folate Deficiency Causes Uracil Misincorporation into Human DNA and Chromosome Breakage: Implications for Cancer and Neuronal Damage,’ Proc. Natl. Acad. Sci. USA 94 (1997): 3290-95.

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